Which cell culture media is carbon dioxide independent?
A eukaryotic growth medium (Program Development Research Group Basal Growth Medium) was developed for CO2-independent maintenance and propagation of human and nonhuman tumor cell lines representing diverse histologies (e.g., cancers of the brain, colon, lung, ovary, and kidney, as well as leukemia and melanoma).
Do HeLa cells need CO2?
Good to know that these cell lines (HEP2 is essentially a batch of HeLa) can grow just at ambient air CO2. Typically, “normal” mammalian cells do need some CO2 as a nutrient (2-5%) to grow but some cell lines can still grow fine without it.
How long can cells survive without CO2?
(Cells can proliferate without CO2 if the medium is buffered with 25 mM HEPES at pH 7.4, but this environment is viable for no more than 10 hours and is highly dependent upon your cell line and cell concentration.)
Is Dmem CO2 independent?
Gibco® CO2 Independent Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York….This CO2 Independent Medium is manufactured as follows:
| With | Without |
|---|---|
| • Phenol Red | • L-glutamine |
| • Sodium pyruvate | • HEPES |
Why does cell culture need CO2?
The purpose of CO2 in cell culture is to maintain a stable physiological pH through the CO2-bicarbonate based buffer system. The atmospheric CO2 can dissolve into cell culture medium, and a small portion of it will react with water to form carbonic acid, which in turn interacts with the bicarbonate ion.
Can you use a CO2 incubator without CO2?
CO2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2 incubator.
Why is 5 CO2 used in cell culture?
5% CO2 for the cell culture is due to the concentration of sodium bicarbonate in the culture medium. 5%CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.
What is in HEPES buffer?
Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent and is categorized as a “Good” buffer which derives from a set of buffers described by Dr. Norman Good and his colleagues in 1966 (Good et.
How does CO2 affect cell culture?
What is the difference between normal incubator and CO2 incubator?
A microbiology incubator keeps organisms at a specific temperature in the ambient air, and a CO2 incubator also balances the pH of the culture.