What type of cells are HEK293T?
HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5).
Where do HEK293T cells come from?
Originating from a female fetus, HEK293 cells are today among the most used mammalian cell lines for a wide range of applications because of their ease of transfection as well as culture. Despite stemming from kidney tissue, HEK293 cells – time and time again – exhibited properties of immature neurons.
What is confluent cell culture?
Confluence （Confluent monolayer）is when the adherent cells cover the adherent surface of the culture vessel. When the adhesive surface of the culture vessel is completely covered with cultured cells, and there is no room for cells to grow as a monolayer, it is called confluent.
Why are HEK293t cells used for transfection?
Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.
Are HEK293t cells immortal?
Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.
What are HT1080 cells?
HT1080 is a fibrosarcoma cell line which has been used extensively in biomedical research. The cell line was created from tissue taken in a biopsy of a fibrosarcoma present in a 35-year-old human male.
What is the morphology of HEK293 cells?
Morphology of HEK293 Cells. The HEK293 cell line is a permanent line established from primary embryonic human kidney, which was transformed with sheared human adenovirus type 5 DNA. The adenoviral genes expressed in this cell line allow the cells to produce very high levels of recombinant proteins.
What is the history of HEK293?
The name HEK293 is thusly named because it was Frank Graham’s 293rd experiment. The type of kidney cell that the HEK293 cell line came arose from is unknown and it is difficult to conclusively characterize the cells post-transformation since adenovirus 5 could have significantly disrupted cell morphology and expression.
Can HEK293T cells form 3-D multicellular spheroids in non-coated polystyrene dishes?
We found that HEK293T cells aggregated and formed into three-dimensional (3-D) multicellular spheroids (MCS) when non-coated polystyrene dishes were used for suspension culture. In particular, the non-coated polystyrene dish from Sumitomo bakelite is the most remarkable characteristic for 3-D MCS among the polystyrene dishes.
Do you offer HEK293 cell lines for high-density suspension culture?
We offer several variants of the HEK293 cell line, including those adapted for high-density suspension culture in serum-free media. For more information, visit the mammalian cell culture pages on our website.