What is the biochemistry of DNA replication?
DNA replication is a semiconservative process, meaning that for every new pair there is one original strand and one new strand. The origin of replication is a sequence of base pairs in the genome where DNA replication begins; these sequences tend to be high in AT content making for easier separation.
What enzyme acts first in DNA replication?
The first step in DNA replication is to ‘unzip’ the double helix structure of the DNA? molecule. This is carried out by an enzyme? called helicase which breaks the hydrogen bonds? holding the complementary? bases? of DNA together (A with T, C with G).
What rules are found in the process of DNA replication?
Replication relies on complementary base pairing, that is the principle explained by Chargaff’s rules: adenine (A) always bonds with thymine (T) and cytosine (C) always bonds with guanine (G).
What happens during DNA replication?
DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. Once the DNA in a cell is replicated, the cell can divide into two cells, each of which has an identical copy of the original DNA.
When and where does DNA replication happen?
Lesson Summary. DNA replication is the process of creating two identical daughter strands of DNA. DNA replication occurs in the nucleus in eukaryotic cells and in the nucleoid region in prokaryotic cells. DNA replication occurs in S phase during the cell cycle prior to cell division.
What are the 7 enzymes involved in DNA replication?
Although the replication mechanisms of these organellar genomes remain unclear in photosynthetic eukaryotes, several organelle-localized enzymes related to genome replication, including DNA polymerase, DNA primase, DNA helicase, DNA topoisomerase, single-stranded DNA maintenance protein, DNA ligase, primer removal …
What is the termination of DNA replication?
Termination of DNA replication occurs when two replication forks meet on the same stretch of DNA, during which the following events occur, although not necessarily in this order: forks converge until all intervening DNA is unwound; any remaining gaps are filled and ligated; catenanes are removed; and replication …
What happens in DNA replication?
Which of the following processes is central to the initiation of DNA replication?
Amongst the array of proteins at the replication fork, DNA polymerases are central to the process of replication. These important enzymes can only add new nucleoside triphosphates onto an existing piece of DNA or RNA; they cannot synthesize DNA de novo (from scratch), for a given template.