What is PBP2 test?
Technical SupportCustomer Service. Thermo Scientific™ Oxoid PBP2′ Latex Agglutination Test is a rapid latex slide agglutination test for detection of PBP2′ as an aid in identifying methicillin resistance in both Staphylococcus aureus (MRSA) and coagulase-negative Staphylococci.
What is PBP2?
Although several observations have documented the importance of penicillin-binding protein 2 (PBP2) in the physiology of Staphylococcus aureus, the precise nature of its role(s) in cell wall synthesis and drug resistance is not well understood. PBP2 is the only bifunctional penicillin-binding protein in S.
Why is it important to test for the PBP2a protein during the latex agglutination test?
The PBP2a test shows poor sensitivity when used directly with positive blood cultures. The gene product of mecA, an altered penicillin binding protein (PBP2a), is the hallmark of methicillin resistance in staphylococci.
Why is latex agglutination testing commonly used?
Latex agglutination testing, also called latex fixation, is a diagnostic study that is widely used as a laboratory method to identify certain antibodies and antigens. The test uses a variety of body fluids including blood, urine, saliva and cerebrospinal fluid, which are dependable on what type of sample is needed.
Why is MRSA so hard to treat?
MRSA infections are more difficult to treat than ordinary staph infections. This is because the strains of staph known as MRSA do not respond well to many common antibiotics used to kill bacteria.
What is PBP and pbp2?
Penicillin-Binding Proteins The three class A PBPs, PBP1a, PBP1b, and PBP2a, are bifunctional enzymes. They polymerize the glycan chains by their N-terminal glycosyltransferase (GTase) domain and perform peptide cross-linking by the C-terminal dd-transpeptidase (TPase) domain.
How can you tell the difference between MSSA and MRSA?
MRSA and MSSA only differ in their degree of antibiotic resistance: other than that there is no real difference between them. Having MSSA on your skin doesn’t cause any symptoms and doesn’t make you ill. You won’t usually know if you have it unless you have a screening test before going into hospital.
What are penicillin-binding proteins and Transpeptidases?
Penicillin-binding proteins (PBPs) are a group of proteins that are characterized by their affinity for and binding of penicillin. They are a normal constituent of many bacteria; the name just reflects the way by which the protein was discovered. PBPs are members of a subgroup of enzymes called transpeptidases.
What does a positive MRSA test mean?
If your MRSA test is positive, you are considered “colonized” with MRSA. Being colonized simply means that at the moment your nose was swabbed, MRSA was present. If the test is negative, it means you aren’t colonized with MRSA.
What is a PBP2 test in microbiology?
Clinical Microbiology. Thermo Scientific™ Oxoid PBP2′ Latex Agglutination Test is a rapid latex slide agglutination test for detection of PBP2′ as an aid in identifying methicillin resistance in both Staphylococcus aureus (MRSA) and coagulase-negative Staphylococci.
What is Oxoid PBP2 test?
Thermo Scientific™ Oxoid PBP2′ Latex Agglutination Test is a rapid latex slide agglutination test for detection of PBP2′ as an aid in identifying methicillin resistance in both Staphylococcus aureus (MRSA) and coagulase-negative Staphylococci.
What is penicillin binding protein (PBP2)?
Penicillin-Binding Protein (PBP2/) Latex Agglutination Test 1. INTENDED USE This test is a rapid latex agglutination assay, detecting PBP2/(also called PBP2a)7, in isolates of Staphylococcus, as an aid in identifying methicillin-resistant Staphylococcus aureus(MRSA) and methicillin- resistant coagulase-negative staphylococci.
Does coagulase-negative staphylococcus test for PBP2’after oxacillin induction?
Two laboratories tested coagulase-negative staphylococci for PBP2′ after induction with oxacillin discs. One laboratory tested 115 methicillin-resistant strains and 45 methicillin-susceptible strains, including 58 fresh clinical isolates. The sensitivity was 96. 5% for testing from TSA blood and 95. 6% for Mueller Hinton Agar.