What is NBT assay?
Nitroblue tetrazolium test is a blood test that measures the ability of the immune system to convert the colorless nitroblue tetrazolium (NBT) to a deep blue. This test is performed as a screen for chronic granulomatous disease (CGD).
Why NBT test is done?
This test is done to screen for chronic granulomatous disease . This disorder is passed down in families. In people who have this disease, certain immune cells do not help protect the body from infections.
What is stimulated NBT test?
Stimulated NBT test in Patients with Chronic Granulomatous Disease. Colorless nitro blue tetrazolium (N.B.T.) is reduced to a water-insoluble formazan which can easily be recognized as a deep blue deposit in cytoplasm. Although the mechanism of reduction of N.B.T. to form formazan in phagocytic neutrophils is uncertain …
What is the color of oxidized nitroblue tetrazolium?
Nitroblue tetrazolium (NBT) is pale yellow in color prior to reduction by superoxide.
How do you read a NBT test?
Normal Results Normally, the white blood cells turn blue when NBT is added. This means that the cells should be able to kill bacteria and protect the person from infections. Normal value ranges may vary slightly from one lab to another. Talk to your doctor about the meaning of your test results.
How do I check my NBT results?
How do you get the NBT test results?
- Click on www.nbt.ac.za.
- Scroll to applicants, then to “NBT Results” then click on “Access your results”
- It will open a new page where you need to click on “Get my NBT results”
- On the new page enter your username which is your ID or passport number.
What is chronic granulomatous inflammation?
Overview. Chronic granulomatous (gran-u-LOM-uh-tus) disease (CGD) is an inherited disorder that occurs when a type of white blood cell (phagocyte) that usually helps your body fight infections doesn’t work properly. As a result, the phagocytes can’t protect your body from bacterial and fungal infections.
How is chronic granulomatous disease diagnosed?
Chronic granulomatous disease (CGD) is diagnosed through: neutrophil function tests to determine how well these white blood cells are functioning. dihydrorhodamine reduction test. genetic testing to confirm the presence of a specific gene mutation that results in CGD.
How do you make nitroblue tetrazolium?
NBT Stock Solution Prepare NBT stock solution by dissolving one NBT tablet in 1 ml of deionized water. BCIP Stock Solution Prepare BCIP stock solution by dissolving one BCIP tablet (25 mg substrate content, Product No. B0274) in 0.5 ml 100% dimethylformamide (DMF, Product No. D4254).
What happens if I fail NBT?
What if I fail? You can’t, strictly speaking, fail the NBTs. Your scores place you in a benchmark (see our website for details) and each university and sometimes, different Faculties within each university, use your scores in a slightly different way.
What is the pass mark for NBT test?
There is no pass mark for the NBTs. Your scores place you in a benchmark band, which assists universities to make decisions about how ready you are for university studies.