What is Morris water maze experiment?
The Morris water maze (MWM) is a test of spatial learning for rodents that relies on distal cues to navigate from start locations around the perimeter of an open swimming arena to locate a submerged escape platform.
How does Morris water maze work?
The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water that is colored opaque with powdered non-fat milk or non-toxic tempera paint, where they must swim to a hidden escape platform.
What is escape latency in Morris water maze?
One of the most commonly used performance measures is the escape latency, defined as the time for the animal to find the platform and escape the maze. Dalm et al.10, showed that the cumulative distance to the platform is correlated with the time spent next to the platform, but not with the escape latency.
What type of memory does Morris water maze test?
A widely used model for studying learning and memory behavior in mice is the Morris water maze (MWM), which specifically assesses spatial learning and memory [11,15].
What is the light dark test?
The light/dark (LD) test is a commonly used rodent test of unconditioned anxiety-like behavior that is based on an approach/avoidance conflict between the drive to explore novel areas and an aversion to brightly lit, open spaces.
What does the T maze measure?
T Maze Spontaneous Alternation is a behavioral test for measuring exploratory behavior in animals, especially rodent models for CNS disorders. This test is based on the willingness of rodents to explore a new environment, i.e. they prefer to visit a new arm of the maze rather than a familiar arm.
Who invented Morris water maze?
Richard Morris
It was developed by Richard Morris at the University of St Andrews in Scotland and first described in two publications in the early 1980s (Morris, 1981; Morris et al., 1982).
What is Y maze test?
Y Maze Spontaneous Alternation is a behavioral test for measuring the willingness of rodents to explore new environments. Rodents typically prefer to investigate a new arm of the maze rather than returning to one that was previously visited.
What is the elevated zero maze?
The elevated plus maze (EPM) and elevated zero maze (EZM) are behavioral tests that are widely employed to assess anxiety-like behaviors in rats and mice following experimental manipulations, or to test the effects of pharmacological agents.
What does a light dark box measure?
The light-dark box is used to test the unconditioned anxiety response of rats or mice. It is based on the innate light aversion and the spontaneous exploratory behavior of rodents in response to mild stressors; the novel environment and the light/open space.
How long it takes a rat to run a maze?
The rats are trained, with the food reward, to run through the maze in less than 10 s without entering the side arm.
How long does it take a rat to learn a maze?
“It takes about nine weeks for a rat to learn the map. We want to use this set-up to investigate how quickly they can take in new knowledge. To understand this, we analyse many trials. In most of them the animals don’t know their starting point, but they do know where the food is.
How do I set up a water maze?
Fill up the water maze with tap water, which should be close to 26°C. This may take several hours, so should be done well in advance. Periodically check the water temperature so that it is within one degree of 26°C. Place the escape platform in the center of the pool. During training, it must be exposed, one inch above the water.
What is the Morris water maze used for?
The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water that is colored opaque with powdered non-fat milk or non-toxic tempera paint, where they must swim to a hidden escape platform.
How do you test a water maze?
Water maze testing. To begin experimental trials with the water maze, fill up the tank so the platform is one inch below the surface of the water. Use non-fat dry milk, or 125 milliliters of non-toxic white tempera paint, to make the water opaque. The lighting and water temperature should be the same as in the training process.
What kind of paint do you use for water maze?
Water maze testing To begin experimental trials with the water maze, fill up the tank so the platform is one inch below the surface of the water. Use non-fat dry milk, or 125 milliliters of non-toxic white tempera paint, to make the water opaque.