What is Lipofectamine used for?
Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection.
What is the difference between Lipofectamine 2000 and 3000?
Lipofectamine 3000 reagent yields higher transfection efficiencies than Lipofectamine 2000 reagent when tested in a variety of cell lines.
What does Cotransfected mean?
transfection
Cotransfection refers to the simultaneous transfection with two separate nucleic acid molecules, such as plasmid DNA and siRNA. Cotransfection is a common procedure for stable transfection. The plasmid DNA may contain a gene that is easily assayed and acts as a marker.
What is Lipofectamine LTX?
Lipofectamine LTX Reagent is a plasmid transfection reagent that offers a balance of potency and gentleness for your cells, resulting in high transfection efficiencies and viabilities.
Is Lipofectamine 2000 toxic to cells?
I have found that in high concentrations, lipofectamine is toxic to HeLa cells, and as previously mentioned, the cell number seeded has an impact on this. I have good results from using DNA to Lipofectamine ratio of 1:1 and 1:2.
Does Lipofectamine expire?
According to ThermoFisher, lipofectamine 3000 can be used until 2 years after the manufacturing date if stored properly (stored in a 4 degree fridge should be fine). The Optimem is sensitive to expiration. I would recommend using the new reagents for your experiment. Hope this helps!
Is lipofectamine 2000 toxic to cells?
What does lipofectamine 3000 reagent do?
Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types (Figure 1) with improved cell viability.
How long do transient transfections last?
24 to 96 hours
Depending on the construct used, transiently expressed transgene can generally be detected for 1 to 7 days, but transiently transfected cells are typically harvested 24 to 96 hours post-transfection.