What is JWH-250?
Unlike many of the older JWH series compounds, this compound does not have a naphthalene ring, instead occupying this position with a 2′-methoxy-phenylacetyl group, making JWH-250 a representative member of a new class of cannabinoid ligands.
What is the difference between the JWH-018 and JWH-250 assays?
The JWH-018 assay had moderate to high cross-reactivity with 17 SC or SC metabolites. The JWH-250 assay had very limited cross-reactivity, with JWH-250-5-OH-pentyl and JWH-250-5-carboxypentyl metabolites at 50% being the only ones with a cross-reactivity >1%, compared to the JWH-250-4-OH calibrator.
Does JWH-018 show up on Elisa?
Arntson et al. (2013) reported two enzyme-linked immunosorbent assays (ELISA) that were designed to detect the 5-OH metabolite of JWH-018 and the 4-OH metabolite of JWH-250 in urine. The JWH-018 assay had moderate to high cross-reactivity with 17 SC or SC metabolites.
What is the difference between Δ9-THC and JWH-018?
The major distinction between Δ 9 -THC and JWH-018 is the efficacy of JWH-018-induced G i/o coupling and adenylyl cyclase inhibition. As depicted, binding of JWH-018 to CB 1 cannabinoid receptors results in marked increases in G i/o protein coupling and inhibition of adenylyl cyclase when compared with the partial agonist Δ 9 -THC.