What is feulgen stain used for?
The Feulgen technique selectively stains DNA, and under controlled conditions, can be used for the photometric determination of DNA content. The reaction consits of two steps.
Why feulgen stain is used for DNA only?
Feulgen stain is a staining technique discovered by Robert Feulgen and used in histology to identify chromosomal material or DNA in cell specimens. It is darkly stained. If the only aldehydes remaining in the cell are those produced from the hydrolysis of DNA, then the technique is quantitative for DNA.
What is Thionin stain?
Thionine is a strongly staining metachromatic dye, which is widely used for biological staining. Thionine can also be used in place of Schiff reagent in quantitative Feulgen staining of DNA. It can also be used to mediate electron transfer in microbial fuel cells.
Which of the following stain is used for DNA and RNA staining?
Oxazole Gold, also known as SYBR® Gold, is the most sensitive fluorescent gel stain available for DNA and RNA.
What is the use of safranin solution?
Safranin-O, also known as basic red 2, is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring all cell nuclei red. It can also be used for the detection of cartilage, mucin and mast cell granules.
What is a nissl stain?
Nissl staining is a standard histological method for visualizing neurons in the brain and spinal cord. Our NeuroTrace 530/615 red fluorescent Nissl stain is selective for the Nissl substance characteristic of neurons and provides more sensitivity than traditional histological dyes like toluidine blue or cresyl violet.
Which chemical substances in the cells can be detected by staining the cells with methyl green and Pyronin?
The combination of methyl green with pyronin can differentiate between RNA and DNA as the DNA appears green and RNA red.
What does Acetocarmine stain?
The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required. To stain chromosomes first acetocarmine dye is prepared using the carmine.