What does tamoxifen do in mice?
Tamoxifen is widely used for the induction of genomic recombination in mice (double-)transgenic for floxed genes and Tamoxifen specific estrogen receptors (ER) coupled to Cre-recombinase (supplemental Table 1) (Hayashi and McMahon 2002).
Is tamoxifen toxic to mice?
Ways to deliver Tamoxifen to Mice Tamoxifen is a hazardous material – make sure to handle it properly. It is also light sensitive, therefore making fresh solutions is recommended.
How long does it take for Cre recombinase to work?
By lipofection, it would take about one day for the Cre to be expressed and then it should be immediately active.
What is the half life of tamoxifen in mice?
The ENDX Cmax, terminal half-life and plasma clearance values were 96 ng/ml, 6.5 h, and 11.8 l/h/kg, respectively.
How does Flox Cre work?
How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.
What is ERT2?
ERT2-Cre-ERT2 fusion protein of a tamoxifen-regulated form of Cre recombinase and two estrogen receptor T2 (ERT2) genes. Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites.
What is a Cre mouse?
A Cre mouse contains a Cre recombinase transgene under the direction of a tissue-specific promoter; a loxP mouse contains two loxP sites that flank a genomic segment of interest, the “floxed” locus. Typically, Cre and loxP mice are produced by using transgenic technology (Nagy 2000).
What is Creer recombinase?
Cre recombinase is a tyrosine recombinase isolated from the bacteriophage P1 that catalyzes the cleavage and ligation of DNA at specific nucleotide recognition sites, loxP sites, via phosphoryl transfer (Hoess et al., 1990). From: Methods in Cell Biology, 2009.
What does tamoxifen do to your brain?
A team from the University of Rochester Medical Center has shown scientifically what many women report anecdotally: that the breast cancer drug tamoxifen is toxic to cells of the brain and central nervous system, producing mental fogginess similar to “chemo brain.”
How do you make tamoxifen solution?
Tamoxifen Injection
- Dissolve 20mg/ml Tamoxifen in corn oil for 1 hr in a roller (hybridization oven) at 65C (protect tube from light with tin foil)
- Keep your stock at +4C for up to a week.
- Before injecting, warm Tamoxifen at 65C for 10min.
What does tamoxifen dissolve in?
Tamoxifen is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of tamoxifen in ethanol and DMF is approximately 20 mg/ml and approximately 2 mg/ml in DMSO. Tamoxifen is sparingly soluble in aqueous buffers.
How do you make a 4-OHT?
For injection of 4-OHT, dissolve 100 mg of 4-OHT powder in 1 mL of ethanol (final concentration of 100 mg/mL), and then dilute the solution 1/10 in peanut or corn oil.