What does lysyl oxidase do?
Lysyl oxidase (LO) is a copper-dependent amine oxidase that plays a critical role in the biogenesis of connective tissue matrices by crosslinking the extracellular matrix proteins, collagen and elastin.
What is the sequence of amino acids that lysyl oxidase specifically acts on?
The mechanism of lysyl oxidase occurs via modification of the ε-amino group of a lysine side chain. The enzyme falls into the category of quinone-containing copper amine oxidases, and the reaction is highly dependent on the cofactor lysyl tyrosylquinone (LTQ).
What is LOX activity?
The LOX activity assay protocol uses a proprietary LOX substrate that releases hydrogen peroxide upon transformation by the LOX present in the sample. When a known concentration of LOX is used, the assay can detect activity from as low as 40 ng of lysyl oxidase in solution.
What is the biochemical reaction catalyzed by lysyl oxidase which is an enzyme essential for Desmosine crosslinking?
Lysyl oxidase catalyzes the oxidative deamination of specific lysyl residues in tropocollagen and tropoelastin. The product, peptidyl-aminoadipic-semialdehyde (allysine), participates in subsequent nonenzymatic condensation reactions.
What is anti lysyl oxidase?
Anti-Lysyl Oxidase (LOX) Antibody is an antibody against Lysyl Oxidase (LOX) for use in Western Blotting, IHC(P). Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Lysyl Oxidase (LOX) in adenocarcinoma cells of human prostate tissues.
What is the cofactor of an enzyme lysyl oxidase?
quinone
The cofactor is a quinone whose structure is derived from the cross-linking of the ϵ-amino group of a peptidyl lysine with the modified side chain of a tyrosyl residue and it has been designated lysine tyrosylquinone (LTQ).
Where is lysyl oxidase located?
Lysyl oxidase proteins (LOXs) are amine oxidases, which are mainly located in smooth muscle cells and fibroblasts and serve an important role in the formation of the extracellular matrix (ECM) in a copper‑dependent manner.
What is the function of lipoxygenase?
Lipoxygenases are non-heme iron enzymes that catalyze the dioxygenation of polyunsaturated fatty acids, yielding hydroperoxides. The first crystal structures have recently been published, revealing an unusual iron site.
How do you increase lysyl oxidase?
Exogenous addition of 1 to 50 ng/ml CTGF to gingival fibroblasts stimulates production of lysyl oxidase enzyme activity up to 1.5-fold after 48 hours, and 50 ng/ml CTGF stimulated insoluble collagen accumulation 1.5- to 2.0-fold after 4, 11, and 18 days of treatment.
Does lysyl hydroxylase require vitamin C?
Vitamin C. Vitamin C, oxygen, ketoglutarate, and iron are essential cofactors for the enzymes prolyl hydroxylase and lysyl hydroxylase, both of which are required for normal collagen metabolism. Vitamin C is important in aerobic metabolism. Fibroplasia is especially sensitive to vitamin C deficiency.
What is cyclooxygenase and lipoxygenase?
Cyclooxygenases and lipoxygenases: the key metabolic enzymes for arachidonic acid and linoleic acid.
What is soybean lipoxygenase?
Soybean seeds contain three lipoxygenase (Lox) enzymes that are controlled by separate genes, Lox1, Lox2 and Lox3. Lipoxygenases play a role in the development of unpleasant flavors in foods containing soybean by oxidation of polyunsaturated fatty acids.