What are the DNA sequences that these two restriction enzymes cut MwoI?
MwoI is a Type II restriction endonucleases enzyme (REase), which specifically recognizes a palindromic interrupted DNA sequence 5′-GCNNNNNNNGC-3′ (where N indicates any nucleotide), and hydrolyzes the phosphodiester bond in the DNA between the 7th and 8th base in both strands.
How can you tell if a site is restrictions?
Restriction sites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes.
How do restriction enzymes recognize a restriction site?
Each restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule—adenine, cytosine, thymine, and guanine). These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA.
What will the restriction enzymes do once it will find its target sequence?
When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule. Typically, the cut is at or near the restriction site and occurs in a tidy, predictable pattern.
Which sequence could a restriction enzyme cut?
When a restriction enzyme finds its restriction site or target sequence (usually a sequence of 4 to 8 nucleotides), it will stop and cleave the DNA molecule (i.e., enzyme digestion). Since the recognition sequence is usually found on both strands (but running in opposite directions), the enzyme will cut both strands.
What is the recognition site for Hind 2?
Hind II recognizes the sequence GTPy/PuAC and generates fragments with blunt ends (1). Compatible ends Hind II generates fragments with blunt ends and is compatible to any other blunt end. Isoschizomers Hind II is an isoschizomer to Hinc II.
What are the recognition and cleavage sites for HindIII?
Endonuclease HindIII is a type II restriction enzyme which recognizes and cleaves the palindromic sequence AAGCTT in the presence of Mg2+. The gene encoding HindIII endonuclease (R) is located upstream of its cognate methyltransferase gene (M).