What are MTMG mice?
These mT/mG mice are useful as a Cre reporter strain; expressing red fluorescence prior to, and green fluorescence following, Cre-mediated recombination in widespread cell and tissue types. The resulting mutant mice were interbred to generate homozygotes prior to arrival at The Jackson Laboratory.
What is a Cre reporter mice?
Abstract. The Cre/loxP system is a strategy for controlling temporal and/or spatial gene expression through genome alteration in mice. As successful Cre/loxP genome alteration depends on Cre-driver mice, Cre-reporter mice are essential for validation of Cre gene expression in vivo.
What are reporter plasmids?
Common reporter genes To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. For bacteria or prokaryotic cells in culture, this is usually in the form of a circular DNA molecule called a plasmid.
What are loxP sites?
LoxP sites are directional 34 bp sequences made up of two 13 bp recognition sites separated by an 8 bp spacer region. The sequences don’t occur naturally in any known genomes other than the P1 bacteriophage, and are long enough that they are unlikely to occur by chance.
What is ubiquitous expression?
For these purposes, ubiquitous expression was defined as the uniformity of expression across tissues regardless of the magnitude of expression whereas tissue-specificity was interpreted as a significant positive deviation from uniformity in one of multiple tissues.
What does Cre stand for in Cre recombinase?
recombination
The P1 gene product was named Cre (causes recombination) and the recombination site was named loxP (locus of crossing (x) over, P1).