What are cell culture flasks?
Cell culture flasks are specifically designed for successful growth and propagation of microbial, insect, or mammalian cells. Most common varieties include flat-sided tissue culture flasks, Erlenmeyer flasks, and spinner flasks.
What are tissue culture treated flasks?
Falcon™ Tissue Culture Treated Flasks. Manufactured from optically clear virgin polystyrene for easy viewing of sample or cells. Corning™ Falcon™ Tissue Culture Treated Flasks features vacuum-gas plasma tissue culture treatment to ensure consistent growth surface.
What is a T75 flask?
T75 flasks for suspension culture of non-adherent cells Made with high quality, optically clear virgin polystyrene, the Thermo Scientific™ Nunc™ Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells.
What is a T25 flask?
T25 flasks for suspension culture of non-adherent cells Made with high quality, optically clear virgin polystyrene, the Thermo Scientific™ Nunc™ Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells. Neck. Surface. Description. Cap.
How much media is in a T25 flask?
T25 flask received a total of 3.0 – 3.5 ml (cells + media volume needed) while a T-25 flask = 9.0 – 10 ml 11. The flask used in the split receives fresh media and is placed back in the incubator. Incubate flasks at 37° C.
What are spinner flasks used for?
Spinner flasks are used as bioreactors for growing suspension cultures in liquid media (Kim et al., 2004). These flasks are stirred tank bioreactors, in which impeller mixing maintains the cells in suspension and the fluid movement helps in mass transport of nutrients and wastes.
How do you treat a flask?
When you get a new flask, you should clean it simply by putting some water inside, putting the cap on, shaking it up a few times, and then removing the cap and dumping the water out. You can do this two or three times if you want, and there is no need to use any type of soap.
How many cells are in a T25 flask?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| T-25 | 156367;156340 | 2.8 x 106 |
| T-75 | 156499;156472 | 8.4 x 106 |
| T-175 | 159910;159920 | 23.3 x 106 |
How many cm2 is a 6 well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Surface area (cm2) | |
|---|---|---|
| Dishes | ||
| 150mm | 150468;168381 | 145 |
| Culture plates | ||
| 6-well | 140675 | 9.6 |
How do I transfer T25 to T75?
If starting with a T25 and transferring to a new T75: Transfer 2.5mL and add 10mL media. Discard remainder. (For fast growth transfer 5mL and add 7mL media). If starting with a T75 and transferring to a new T75: Transfer 0.8mL for a 1/6 split and add 11mL media.
What is the best cell culture flask for suspension cells?
Cell culture flasks for suspension cells. Nunc EasYFlasks non-treated cell culture flasks. Made with high quality, optically clear virgin polystyrene, these non-treated cell culture flasks offer a high level sterility assurance ideal for suspension culture of non-adherent cells.
Why do we need to subculture cells?
In cell culture there is frequently the need to subculture cells. In doing so, cells can be propagated for the purposes of increasing cell numbers or providing cells in a culture vessel suitable to one’s needs. There are a number of ways to remove cells from one culture vessel and pass them to another vessel.
Why choose Lonza for cell culture?
Cell Culture Technical Information Lonza offers an extensive line of cell culture media and reagents backed by years of experience and innovation.
What are the different cell culture techniques?
There are two cell culture techniques to grow cells in culture, as monolayers on an artificial substrate (i.e., adherent culture) or free-floating in culture medium (suspension culture).