Is resazurin toxic to cells?
Resazurin, introduced as a cell viability indicator under the trade name alamarBlue(®), is generally regarded as nontoxic when used according to manufacturer’s suggested shorter-term incubation time specifications. However, problems arise when exposure times are extended to longer-term cultures on the order of days.
How does the resazurin test work?
Resazurin gives milk a characteristic blue colour and the test is based on the ability of bacteria in the milk to reduce the blue dye.
What is resazurin used for?
Resazurin is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.
Is Alamar Blue toxic to cells?
alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation. If necessary, longer incubation times may be used for greater sensitivity without compromising cell health.
Is Alamar Blue toxic?
Resazurin, the active ingredient of alamarBlue reagent, is a non-toxic, cell-permeable compound that is blue in color and virtually non-fluorescent. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent.
How is resazurin assay used to measure cell viability?
The amount of resorufin can be monitored by measuring fluorescence or absorbance, which is proportional to the number of living cells in the sample. Depending on the cell types, the resazurin assay can be used to detect as few as 40 cells with reproducible and sensitive signal. Figure 1. The principle of resazurin cell viability assay.
Is the resazurin metabolism assay a suitable screening test for eye irritation?
The nontoxic, nonradioactive resazurin metabolism assay allows rapid assessment of many samples with simple equipment and at reduced cost for continuous monitoring of corneal viability. This assay seems to be suitable as a toxicological screening test for eye irritation determination.
What are the advantages of resazurin reduction assay?
The major advantages of the resazurin reduction assay are that it is relatively inexpensive, uses a homogeneous format, and is more sensitive than tetrazolium assays ( Riss et al., 2013 ).
What is the purpose of the resorufin assay?
Such assays are widely used for investigating cell response to a given agent, drug or to establish relative cytotoxicity of chemicals. Living cells maintain a reducing environment within their cytoplasm and mitochondria, in which resazurin (blue and non-fluorescent) is reduced by dehydrogenase enzymes to form the red fluorescent dye resorufin.