How does dihydrofolate reductase involved in purine synthesis?
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a proton shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids.
What type of enzyme is dihydrofolate reductase?
Dihydrofolate reductase is an enzyme that converts dihydrofolate to tetrahydrofolate and is involved in purines and thymidylate synthesis. It is encoded by the human DHFR gene. Antifolate drugs, methotrexate (MTX) and trimetrexate, can tightly bind to DHFR and inhibit DNA synthesis and cell proliferation.
Which reaction directly produces dihydrofolate?
DHFR catalyzes the two-step reduction of folic acid, first to dihydrofolate and then to the active coenzyme THF (Fig. 39.1). The enzyme is also responsible for the conversion of dihydrofolate, which is produced in the reaction of thymidine monophosphate biosynthesis, to THF (Fig. 39.1).
Is folic acid a substrate for dihydrofolate reductase?
Folic Acid as Substrate for DHFR. Due to the slow rate of DHFR with FA, activity with this substrate was measured only by the HPLC method.
What inhibits bacterial dihydrofolate reductase?
Pyrimethamine and trimethoprim are the most widely used dihydrofolate reductase inhibitors. Pyrimethamine is a known terattogen but is more effective then trimethoprim, which is usually administered in conjunction with a dihydropteroate synthase inhibitor (sulfamethoxazole).
Which immunosuppressant drug binds to dihydrofolate reductase and prevent synthesis of tetrahydrofolate?
Methotrexate inhibits dihydrofolate reductase (DHFR), an enzyme responsible for the conversion of dihydrofolate into tetrahydrofolate, thus inhibits cell proliferation and growth.
Which of the following is an inhibitor of dihydrofolate reductase?
Methotrexate and pralatrexate are dihydrofolate reductase inhibitors that deplete cellular tetrahydrofolate cofactors.
What is dihydrofolate reductase?
Dihydrofolate reductase is an enzyme that converts dihydrofolate to tetrahydrofolate and is involved in purines and thymidylate synthesis. It is encoded by the human DHFR gene. Antifolate drugs, methotrexate (MTX) and trimetrexate, can tightly bind to DHFR and inhibit DNA synthesis and cell proliferation.
What is the role of DHFR in the synthesis of tetrahydrofolate?
Dihydrofolate reductase (DHFR) is a ubiquitous enzyme, which plays an essential role in the biosynthesis of tetrahydrofolate cofactors. It catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate (see Fig. 1 under comments).
What is the function of dihydrofolate?
Dihydrofolate reductase is an enzyme that converts dihydrofolate to tetrahydrofolate and is involved in purines and thymidylate synthesis. It is encoded by the human DHFR gene.
Is human dihydrofolate reductase bound to NADP or NADP?
human dihydrofolate reductase is bound to NADP. Constituents of the folate cycle could be involved in the etiology of idiopathic intellectual disability.