Can GFP antibody detect YFP?
YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Aequorea victoria. Recognizes native and denatured forms of jellyfish GFP and its variants such as: EGFP, YFP, EYFP, and CFP.
What is an anti-GFP antibody?
The target antigen refers to green fluorescent protein (GFP), commonly used as an epitope tag for recombinant proteins for detection via immunofluorescence, flow cytometry or western blot. …
Does GFP antibody recognize Venus?
Venus, GFP, EGFP and YFP from Aequorea victoria. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins. Polyclonal antibody is supplied as 100 uL (3mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Does GFP antibody recognize BFP?
Our GFP antibody does cross-react with the many fluorescent proteins that are derived from the jellyfish Aequorea victoria. These are all proteins that differ from the original GFP by just a few point mutations (EGFP, YFP, mVenus, CFP, BFP etc.).
Can GFP antibody detect CFP?
Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.
How many KDA is GFP?
26.9 kD
With GFP, the primary structure is 239 amino acids with a total molecular weight of 26,870 daltons, or 26.9 kD.
How many kDa is GFP?
What is the purpose of immunofluorescence?
Immunofluorescence (IF) is an important immunochemical technique that allows detection and localization of a wide variety of antigens in different types of tissues of various cell preparations.
Can GFP antibody detect RFP?
A: The anti-GFP antibody was made using the N-terminal of GFP as an antigen and will not react with RFP proteins dsRED or mCherry, since their sequences are so different.
How long does GFP fluorescence last?
The half-life of the green fluorescent protein (GFP) was determined biochemically in cultured mouse LA-9 cells. The wild-type protein was found to be stable with a half-life of ~26 h, but could be destabilized by the addition of putative proteolytic signal sequences derived from proteins with shorter half-lives.