What is double floxed?
The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated recombination. Since its first publication, numerous FLEX switch plasmids have been generated.
What does it mean if a gene is floxed?
Floxing a gene allows it to be deleted (knocked out), translocated or inserted (through various mechanisms in Cre-Lox recombination). The floxing of genes is essential in the development of scientific model systems as it allows spatial and temporal alteration of gene expression.
How do you get floxed mice?
Traditionally, flox mice have been obtained by gene targeting in embryonic stem cells followed by production of germline chimeric mice.
What is Dio in virus?
The double-floxed inverse open reading frame (DIO) construct comprises inverted terminal repeats (ITR), the EF1α promoter, an eYFP-ChR2 fusion gene surrounded by a pair of LoxP sites and a pair of Lox2722 sites oriented inward, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and a human …
What does Flox mean in mice?
The Cre-lox system allows for the deletion of a gene in mouse ES cells and its inversion or complete inactivation. This process is called “floxing” since it combines flanking the gene with the help of its adjacent two loxP sites and the Cre-lox recombination system.
How do you know if you have been floxed?
Signs + Symptoms Mitochondrial dysfunction and DNA damage. Brain Fog. Peripheral Neuropathy. Blurry Vision.
What are LoxP sites?
LoxP sites are directional 34 bp sequences made up of two 13 bp recognition sites separated by an 8 bp spacer region. The sequences don’t occur naturally in any known genomes other than the P1 bacteriophage, and are long enough that they are unlikely to occur by chance.
What is Dio cre?
Principle of Cre-DIO (double-floxed inverse open reading frame) system. Without Cre, the GOI and reporter gene are inverted and silenced. In the presence of Cre, first, the transgene will be reoriented, then one of the two lox sites in each pair will be excised.