What is the function of PBP2?
PBP2 is the only bifunctional penicillin-binding protein in S. aureus (3, 8), and the transpeptidase (TPase) domain of the protein was found to be essential for the growth and survival of the bacteria (14, 17).
What is PBP and PBP2?
Penicillin-Binding Proteins The three class A PBPs, PBP1a, PBP1b, and PBP2a, are bifunctional enzymes. They polymerize the glycan chains by their N-terminal glycosyltransferase (GTase) domain and perform peptide cross-linking by the C-terminal dd-transpeptidase (TPase) domain.
What is PBP2a?
PBP2a is a unique transpeptidase that is not inhibited well by β-lactam antibiotics. Hence, it is able to continue peptidoglycan crosslinking in the face of the challenge by these antibiotics, when other PBPs with transpeptidase activity are inhibited.
What does PBP2 negative mean?
A negative reaction with both Test and Control Latex indicates that the organism contains. no PBP2′ and should be reported as a presumptive methicillin-sensitive Staphylococcus aureus (MSSA).
What is penicillin binding protein 2?
Presence of the protein penicillin binding protein 2A (PBP2A) is responsible for the antibiotic resistance seen in methicillin-resistant Staphylococcus aureus (MRSA). The β-lactam ring is a structure common to all β-lactam antibiotics.
What is MRSA?
Methicillin-resistant Staphylococcus aureus (MRSA) is a cause of staph infection that is difficult to treat because of resistance to some antibiotics. Staph infections—including those caused by MRSA—can spread in hospitals, other healthcare facilities, and in the community where you live, work, and go to school.
What is Transpeptidation reaction?
Transpeptidation reaction is the chemical reaction that forms the peptide cross-links or bonds during the synthesis of peptidoglycan (murein) in a bacterial cell. These processes culminate to the formation of peptidoglycan (murein) layer in both Gram-positive and Gram-negative bacteria.
What do Autolysins do?
Autolysins are endogenous lytic enzymes that break down the peptidoglycan components of biological cells which enables the separation of daughter cells following cell division.
What is cassette chromosome?
A gene cassette is a type of mobile genetic element that contains a gene and a recombination site. Each cassette usually contains a single gene and tends to be very small; on the order of 500–1000 base pairs. They may exist incorporated into an integron or freely as circular DNA.
Does all MRSA have mecA gene?
Although the mecA gene is present in all MRSA isolates, the phenotypic expression of methicillin resistance is more variable. For example, S. aureus isolates grown at 32° C, rather than at 37° C, are more likely to express methicillin resistance.
What is PBP2 test used for?
This test is a rapid latex agglutination assay, detecting PBP2′ (also called PBP2a), in isolates of Staphylococcus, as an aid in identifying methicillin-resistant Staphylococcus aureus (MRSA). This kit must be stored at 2-10°C.
What is penicillin binding protein (PBP2)?
Penicillin-Binding Protein (PBP2/) Latex Agglutination Test 1. INTENDED USE This test is a rapid latex agglutination assay, detecting PBP2/(also called PBP2a)7, in isolates of Staphylococcus, as an aid in identifying methicillin-resistant Staphylococcus aureus(MRSA) and methicillin- resistant coagulase-negative staphylococci.
What is PBP 2 in MRSA?
MRSA REAGENT (PBP 2′) Rapid Test (CDL-C4310) This test is a rapid latex agglutination assay, detecting PBP2′ (also called PBP2a), in isolates of Staphylococcus, as an aid in identifying methicillin-resistant Staphylococcus aureus (MRSA).
What is the Oxoid PBP2/test?
The Oxoid PBP2/ test has previously been evaluated worldwide, demonstrating its high sensitivity and specificity4,5,11. Latex particles sensitised with a monoclonal antibody against PBP2/ will specifically react with methicillin-resistant staphylococci to cause agglutination visible to the unaided eye. 3. COMPONENTS OF THE KIT