What is immunoelectrophoresis serum?
Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are proteins that function as antibodies, which fight infection. There are many types of immunoglobulins that fight different types of infections.
What are the uses of serum immunoelectrophoresis?
The immunoelectrophoresis-serum test (IEP-serum) is a blood test used to measure the types of Ig present in your blood, especially IgM, IgG, and IgA.
Does serum contain IgG?
IgG1 in serum (stabilization of disulfide bonds). Depending on the therapeutic target of the antibody, mutations in the Fc region can be carried out to either enhance or reduce effector functions. A total of 16 disulfide bonds (4 inter-chain and 12 intra-chain) are found in IgG1 molecules (Fig. 1).
What does IgG mean in medical terms?
Immunoglobulin G (IgG) is the most common type. IgG has 4 different subclasses, IgG1— 4. IgG is always there to help prevent infections. It’s also ready to multiply and attack when foreign substances get into the body. When you don’t have enough, you are more likely to get infections.
How do you separate serum from IgG?
Selective precipitation with electrodialysis and zinc acetate precipitation appears to be an effective technique for the separation of IgG and IgM from albumin in citrated plasma.
What is the structure of IgG?
Structure. IgG antibodies are large globular proteins with a molecular weight of about 150 kDa made of four peptide chains. It contains two identical γ (gamma) heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus a tetrameric quaternary structure.
Why serum protein electrophoresis is done?
Why It Is Done Serum protein electrophoresis is most often done to help diagnose and monitor a wide variety of conditions. These include: Some forms of cancer. Problems with the kidneys or liver.
How is immunoelectrophoresis performed?
Immunoelectrophoresis is performed by placing serum on a slide containing a gel designed specifically for the test. An electric current is then passed through the gel, and immunoglobulins, which contain an electric charge, migrate through the gel according to the difference in their individual electric charges.
What is IgG IgA IgM IgD IgE?
In normal serum, about 80% is immunoglobulin G (IgG), 15% is immunoglobulin A (IgA), 5% is immunoglobulin M (IgM), 0.2% is immunoglobulin D (IgD), and a trace is immunoglobulin E (IgE). Elevations of IgG, IgA, and IgM may be due to polyclonal immunoglobulin production.
What are immunoglobulins (Igs)?
Immunoglobulins (Igs) are a group of proteins also known as antibodies. Antibodies provide your body with the first line of defense against invading pathogens. Immunoglobulins can be described as either normal or abnormal.
What is the normal range of IgG IgA IgM IgD?
Clinical Information. In normal serum, about 80% is immunoglobulin G (IgG), 15% is immunoglobulin A (IgA), 5% is immunoglobulin M (IgM), 0.2% is immunoglobulin D (IgD), and a trace is immunoglobulin E (IgE). Elevations of IgG, IgA, and IgM may be due to polyclonal immunoglobulin production.
What is an abnormal IgG?
Abnormal Igs also suggest the presence of disease. An example of an abnormal Ig is monoclonal protein, or M protein. The immunoelectrophoresis-serum test (IEP-serum) is a blood test used to measure the types of Ig present in your blood, especially IgM, IgG, and IgA. The IEP-serum test is also known by the following names: