What is Fluoro-Jade B?
Abstract. Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. However, Fluoro-Jade B has an even greater specific affinity for degenerating neurons.
What does Fluoro-Jade B stain?
Fluoro-Jade is a fluorescent derivative used for histological staining of degenerating neurons. This technique is simple and sensitive enough to label distal dendrites, axons, axon terminals as well as neuronal bodies.
How does fluoro-Jade C work?
Fluoro-Jade® C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Reagent detects degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain.
What does FJB stain?
Fluoro-Jade B (FJB) is an anionic fluorescein derivative that has been reported to specifically stain degenerating neurons.
What does fluoro-Jade C stain?
Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons.
How is the staining solution for fluoro-jade B made?
The staining solution was prepared from a 0.01% stock solution for Fluoro-Jade® B that was made by adding 10 mg of the dye powder to 100 mL of distilled water. To make up 100 mL of staining solution, 4 mL of the stock solution was added to 96 mL of 0.1% acetic acid vehicle. This results in a final dye concentration of 0.0004%.
What is fluoro-jade B?
Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration.
Why time window is critical for fluoro jade-B staining?
Hi Kaliappan, time window is critical for Fluoro Jade-B staining because Fluoro Jade is used to label dying or dead neurons in brain tissues. If you collect and fix tissues too early or too late, you have fewer chances to get positive staining. This is not a complex technique.
What is the PMID for fluro-Jade B staining?
PMID 14651809. ^ Damjanac, M., Rioux Bilan, A., Barrier, L., Pontcharraud, R., Anne, C., Hugon, J., Page, G. (2007). “Fluro-jade B staining as useful tool to identify activated microglia and astrocytes in a mouse transgenic model of Alzheimer’s disease”.