Why is Lactophenol cotton blue stain used in fungal staining?
Fungal Stains. Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls.
What is the advantage of the observing a culture stained with Lactophenol cotton blue?
Phenol kills fungus. Lactic acid acts as a clearing agent and helps preserve the fungal structures. Cotton blue is an aniline dye that stains the chitin in the fungal cell walls which adds colour to the fungal preparation thereby enhancing and contrasting the structures.
What are the components of Lactophenol?
Lactophenol Cotton Blue (LPCB).
- Cotton blue (Aniline blue) 0.05 g.
- Phenol crystals (C6H5O4) 20 g.
- Glycerol 40 mL.
- Lactic acid (CH3CHOH COOH) 20 mL.
- Distilled water 20 mL.
Why Koh is used in fungal diagnosis?
These scrapings are then mixed with potassium hydroxide. The potassium hydroxide destroys the healthy skin cells, leaving behind only fungal cells. Normal results of a KOH test will show no fungi present, while abnormal results will tell your doctor that you may have a fungal infection.
What does lactophenol blue stain?
Principle of Lactophenol cotton blue Staining Methyl blue is a histological stain which stains collagen blue in tissue sections, and lactophenol is a mixer of four components such as phenol, lactic acid, and glycerol in water.
What does Lactophenol blue stain?
How do you stain fungi with Lactophenol cotton blue?
Lactophenol Cotton Blue is used as staining solution for fungi. 1) Place a drop of Lactophenol Cotton Blue reagent on a clean and dry slide. The stain imparts a blue colouration on hyphae. 2) By using a nichrome inoculating wire, carefully tease the fungal culture, into a thin preparation.