What is the highest DNA band size in 1 kb DNA ladder?
Description: The 1 Kb Plus DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 12 kb. The ladder contains a total of twenty bands: twelve bands ranging in size from 1000 bp to 12,000 bp in 1000-bp increments and eight bands ranging in size from 100 to 1650 bp.
What is a 100 bp ladder?
Quick-Load® 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye. The digested DNA includes fragments ranging from 100-1,517 base pairs.
What is a 1 kb DNA size standard ladder?
1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).
What is the composition of the 1 KB plus DNA ladder?
The 1 kb PLUS TM is composed of 14 individual DNA fragments: 25K, 10K, 8K, 6K, 5K, 4K, 3K, 2.5K, 2K, 1500, 1000, 750, 500, 250 base pairs. This product contains two enhanced bands (3K and 1K bp) for easy reference. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.
What is a 100 bp DNA ladder used for?
A 100 bp DNA ladder is a DNA size standard used for the sizing and quantification of double-stranded DNA of the size in the range of 100 bp to 1500 bp. These ladders consist of about 11 highly purified DNA fragments that form separate clear bands for the identification of other DNA fragments within that range.
What are DNA ladders and how are they made?
Commercially available DNA ladders come in the 50 bp, 100 bp, 1000 bp, and 3000 bp form. These ladders are created by the digestion of known-length DNA fragments from natural sources by the restriction enzymes. The length of the fragments, thus, dependent on the restriction enzyme being used, this makes the process not entirely controllable.
What is the recommended load for electrophoresis using 1 KB ladder?
1 kb ladder can be bought commercially at various concentrations, but the recommended load for an electrophoresis run is 0.5 µg (5µl). These can be used in either agarose or in polyacrylamide gels with the concentration of gel at 0.75% to 1%. These ladders come with different tracking dyes like bromophenol blue, xylene cyanol FF.