How does LiCor Odyssey work?
How the LI-COR Odyssey Platform Works. Using infrared fluorescent signals that are directly proportional to the amount of target protein, LI-COR Odyssey can accurately and reproducibly measure changes in protein levels via ratiometric detection.
What is LiCor imaging?
The LiCor Odyssey Infrared Imaging System 9120 gives your lab clear sharp digital results without the background fluorescence. The Odyssey Imaging System 9120 features a wide linear range, two-color detection and quantification, high sensitivity, accurate detection of strong or weak signals, and nir fluorescence.
What is Odyssey Imaging System?
The Odyssey Fc System is the only imaging system that offers superior chemiluminescent detection and the benefits of quantitative infrared Western blot detection as well as the ability to document DNA gels using the 600nm channel.
How do you use an Odyssey scanner?
34 second clip suggested1:33Using the LI-COR Odyssey Scanner to image the Q-Plex Array – YouTubeYouTubeStart of suggested clipEnd of suggested clipWith 70% ethanol and a kimwipe place the plate on the front left corner of the scanning bed. AndMoreWith 70% ethanol and a kimwipe place the plate on the front left corner of the scanning bed. And close the door press scan in the software. When you are ready to scan the plate.
How do you use Western blot Odyssey?
10 Tips for Reproducible Odyssey® Western Blots
- Use the Right Membrane.
- Dry Membrane after Transfer.
- Optimize Blocking Conditions.
- Optimize the Dilution of Secondary Antibodies.
- Validate Primary Antibodies.
- Determine the Combined Linear Range of Detection.
- Use Proper Experimental Controls.
What is in Odyssey blocking buffer?
Odyssey Blocking Buffer (PBS) is a ready-to-use formulation in phosphate-buffered saline that provides optimal blocking conditions for antibodies requiring PBS- based buffer systems. The blocker does not contain mammalian proteins.
Why do you need methanol in transfer buffer?
The presence of methanol in the transfer buffer serves two main purposes: It promotes dissociation of SDS from the protein and dramatically improves adsorption of proteins onto membranes in the presence of SDS, although these effects may vary with proteins.
How long can you incubate secondary antibody?
3.2 Interaction between primary antibodies and secondary antibody might take more than 48 hours to reach its maximum. Incubation with secondary Abs for one hour is a universally used standard protocol in western blotting.
What is in Licor blocking buffer?
Formulation. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.
Can I use ethanol instead of methanol in transfer buffer?
In many cases, ethanol can be substituted for methanol in the transfer buffer with minimal impact on transfer efficiency. Increasing methanol in the transfer buffer decreases protein transfer from the gel and increases binding of the protein to nitrocellulose membrane.