How does Tol2 system work?
The Tol2 element is an autonomously active transposon, containing a gene encoding a complete and functional transposase that is capable of identifying, excising, and reinserting the DNA element defined by its inverted terminal repeats (ITR) or other elements with the same ITRs.
What is Tol2 transposon system?
The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The Tol2 transposon system has been shown to be active in all vertebrate cells tested thus far, including zebrafish, Xenopus, chicken, mouse, and human.
How do transposase work?
Transposase is an enzyme that binds to the end of a transposon and catalyses its movement to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism. The repositioning of these transposons (which coded for color) allowed other genes for pigment to be expressed.
What are the two mechanisms of mobility of transposons?
Class II (DNA Transposons) move by one of two mechanisms. In the cut-&-paste pathway, the transposon leaves one locus and integrates at another. In the replicative pathway, the original transposon remains in place while new copies are mobile.
Why is there a need to co inject mRNA that encodes transposase?
However, in zebrafish the most common way to supply the transposase is by co-injection of mRNA encoding the transposase (Fig. 1F). This insures that the transposase is only available transiently and that the transposon integrations will be stable following natural mRNA degradation.
What type of enzyme is transposase?
Transposases are enzymes that identify the inverse terminal repeat sequences within the DNA and proceed to bind and excise the DNA transposons in between the terminals.
Which of the following function is not performed by transposase?
Which of the following functions is not performed by transposase? Explanation: The transposase performs three functions in the transposition of the IS element. First, it precisely excises the IS element from the transposon. Second, it makes staggered cut in the target DNA segment.