What are the 4 restriction enzymes?
Traditionally, four types of restriction enzymes are recognized, designated I, II, III, and IV, which differ primarily in structure, cleavage site, specificity, and cofactors.
How many types of restriction enzymes are there?
Today, scientists recognize three categories of restriction enzymes: type I, which recognize specific DNA sequences but make their cut at seemingly random sites that can be as far as 1,000 base pairs away from the recognition site; type II, which recognize and cut directly within the recognition site; and type III.
How many enzymes are used in restriction digest?
In general, we recommend 5–10 units of enzyme per µg DNA, and 10–20 units for genomic DNA in a 1 hour digest.
What are DNA restriction enzymes?
A restriction enzyme is an enzyme isolated from bacteria that cuts DNA molecules at specific sequences. The isolation of these enzymes was critical to the development of recombinant DNA (rDNA) technology and genetic engineering.
What type of enzyme is restriction endonuclease?
DESCRIPTION AND GENERAL NOTES. Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. Type I REs are important in bacterial function but do not cleave DNA at specific sequences.
What is SDAI (SBFI) restriction enzyme?
Thermo Scientific SdaI (SbfI) restriction enzyme recognizes CCTGCA^GG sites and cuts best at 37°C in its own unique buffer.
What is the replacement for SBFI-HF?
This product has been replaced with the high fidelity version, SbfI-HF™ ( NEB #R3642 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
Is SBFI a one unit enzyme?
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. SbfI is an isoschizomer of Sse8387I. Not sensitive to CpG, dcm, or dam methylation. Is SbfI a Time-Saver™ Qualified enzyme? Is SbfI activity sensitive to dam, dcm or mammalian CpG methylation?
Where can I find more information about BSA-free Restriction enzymes?
Find more details at www.neb.com/BSA-free. High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer ( rCutSmart ™ Buffer ). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).