What does phalloidin do to cells?
Phalloidin binds F-actin, preventing its depolymerization and poisoning the cell. Phalloidin binds specifically at the interface between F-actin subunits, locking adjacent subunits together.
What is rhodamine phalloidin?
Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC). Also available as a room-temperature-stable, ready-to-use solution: ActinRed 555 Ready Probes Reagent.
Do you need to permeabilize cells for phalloidin?
Even a small amount of triton is sufficient to permeabilise for phalloidin. Acetone fixing works well but phalloidin will not stain methanol fixed cells. You can use 0.1% triton X (very mild) for 15-20 mins at RT in order to permeabilise the cells as phalloidin will not penetrate without permeabilisation.
How does phalloidin affect hepatocytes?
Phalloidin increases F-actin microfilament content and actin-directed immunofluorescence in hepatocytes in vivo and also increases actin polymerization and the stability of F-actin in vitro.
What is FITC phalloidin?
Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC). • Selectively stains F-actin. • Excitation/Emission: 496/516 nm. • Superior to antibody staining. • Optimal for fixed and permeabilized samples.
Is phalloidin a primary or secondary antibody?
Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during the primary or secondary antibody incubation step.
What happens to hepatocytes exposed to phalloidin?
When present in the blood, phalloidin and other phallotoxins are selectively taken up by hepatocytes. With respect to the strong organotropism of phallotoxins, intravenously injected phalloidin binds preferentially to microfilamentous F-actin of hepatocytes.