What is the pKa of imidazole?
6.8
Imidazole is an important intracellular buffer because it is a building block for proteins and enzymes and because it has a pKa (at 37°C) of 6.8 (close to intracellular pH).
Is imidazole soluble in DMF?
Imidazole is an organic compound with the formula C3N2H4. It is a white or colourless solid that is soluble in water, producing a mildly alkaline solution….Imidazole.
| Names | |
|---|---|
| Density | 1.23 g/cm3, solid |
| Melting point | 89 to 91 °C (192 to 196 °F; 362 to 364 K) |
| Boiling point | 256 °C (493 °F; 529 K) |
| Solubility in water | 633 g/L |
Is imidazole a functional group?
Histidine, an essential amino acid, has as a positively charged imidazole functional group. The imidazole makes it a common participant in enzyme catalyzed reactions. The unprotonated imidazole is nucleophilic and can serve as a general base, while the protonated form can serve as a general acid.
Which amino acids contain imidazole ring?
Histidine, an essential amino acid, has as a positively charged imidazole functional group. The imidazole makes it a common participant in enzyme catalyzed reactions.
How does imidazole affect my quantitation of protein?
How does imidazole affect my quantitation of protein? Since imidazole absorbs UV radiation at 280 nm, an elution profile measured at 280 nm while purifying a 6xHis tagged protein by FPLC will show an increase in absorbance above the background signal allowing quantitation of your protein.
How to pronounce “imidazole”?
Pronunciation of imidazoles with 1 audio pronunciations 0 rating rating ratings Record the pronunciation of this word in your own voice and play it to listen to how you have pronounced it.
Is imidazole more acidic than pyrrole?
Thus imidazole can act as acid and base. It is more acidic than pyrrole and more basic than pyridine. (e) Reaction with aldehydes and ketones: N-unsubstituted imidazole undergoes hydroxymethylation at C4-position when treated with HCHO (formaldehyde) in the presence of DMSO.
What is PKA enzyme?
Protein kinase A [EC 2.7.11.11; PKA; cyclic adenosine monophosphate (i.e., cyclic AMP)-dependent protein kinase; cAPK] is a “serine protein kinase”, an enzyme that targets serine and threonine residues. It constitutes a subgroup of a superfamily of over 2000 vertebrate protein kinases.