What is Folin reagent used for?
Folin’s reagent or sodium 1,2-naphthoquinone-4-sulfonate is a chemical reagent used as a derivatizing agent to measure levels of amines and amino acids. The reagent reacts with them in alkaline solution to produce a fluorescent material that can be easily detected.
What is Folin’s test?
[ fō′lĭnz ] n. A spectrophotometric test used to determine the quantity of uric acid in urine in which a phosphotungstic acid is mixed with a base. A test used to determine the quantity of urea in urine in which urea is decomposed by boiling with magnesium chloride and the freed ammonia is measured.
What is the purpose of Lithium Li ions in the Folin Ciocalteu reagent?
Folin–Ciocalteu Assay The FCR (Folin and Ciocalteu, 1927) provides an improvement over the Folin–Denis reagent with the addition of lithium sulfate to the reagent. The lithium salts reduce the amount of precipitate that can form when high concentrations of reagent are used to increase the assay’s reactivity.
How do you make Folin Denis reagent?
Folin-Denis′ reagent can be prepared by mixing mixture of sodium tungstate and (phospho)molybdic acid in phosphoric acid. This reagent is generally used in determination of phenolics in urine.
What is the composition of Folin reagent?
The Folin-Ciocalteu (F-C) Assay was developed in 1927 for the measurement of tyrosine (1). The reagent consists of a mixture of sodium molybdate, sodium tungstate and other reagents. Upon reaction with phenols, it produces a blue color which absorbs at 765 nm.
What is the name of the product produced in the Folin-Ciocalteu assay and how do you measure it?
What is Folin Ciocalteu reagent made of?
What is Folin-Ciocalteu reagent made of?
What is Folin-Ciocalteu reagent justify the name of the reagent?
Folin & Ciocalteu′s phenol reagent has been used for the estimation of total phenolic content in samples.
What is the principle of Lowry method?
Principle: The principle involved in Lowry method is determining the protein concentration by calculating the reactivity of the peptide nitrogen with the Copper ions under alkaline conditions followed by reduction reaction of Folinciocalteay phosphomolybdic phophotungstic acid to Heteropolymolybdenum blue by copper …
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