How do you DEPC treat water?
DEPC Treated Water Recipe
- Add 1ml of 0.1% Diethylpyrocarbonate (DEPC) to 1000ml distilled water.
- Mix well and let set at room temperature for 1 hour.
- Autoclave.
- Let cool to room temperature prior to use.
What is DEPC and how it works?
Diethyl pyrocarbonate (DEPC) is an efficient, nonspecific inhibitor of RNases. It is typically used to treat water and solutions before working with easily degraded RNA. DEPC reacts with amine, hydroxy and thiol groups of proteins thereby inactivating RNAses (and other enzymes).
Does DEPC treated water expire?
DEPC treatment is an effective way to eliminate RNAse activity….Specification Test Results.
| Recipe | 0.01ml DEPC |
|---|---|
| Shelf Life | 3 Years (from Date of Manufacture) |
| Sterility | SAL 10-6 |
| Endotoxins | 0.005eu/ml |
| Activity | None Detected |
Is DEPC toxic?
1. DEPC is toxic and not appropriate in a lab where it cannot be handled properly. This objection is with merit, as improper handling may present a serious health risk. DEPC is too difficult to remove from reagents and interferes with PCR and other re-actions.
Can DEPC water go down drain?
Refer to individual constituents. Refer to data for ingredients, which follows: DEPC TREATED WATER: ■ DO NOT discharge into sewer or waterways. All waste must be handled in accordance with local, state and federal regulations.
Is DEPC treated water toxic?
1. DEPC is toxic and not appropriate in a lab where it cannot be handled properly. This objection is with merit, as improper handling may present a serious health risk.
Can I use DEPC treated water for Qpcr?
DEPC treatment would be preferred for enhance sensitivity and completely remove any potential for RNA degradation by RNases during cDNA synthesis, but nuclease free water should be sufficient for PCR. However, DEPC treatment will not affect your PCR, if you choose to use this, as long as the DEPC has been inactivated.
Is DEPC a carcinogen?
Diethyl pyrocarbonate (DEPC) DEPC is a potent, non-specific chemical inhibitor of RNase2 and its most common application has been to make water nuclease-free. DEPC is also carcinogenic and should be handled with care and with strict attention to the safety recommendations of the manufacturer.