How do you test for vitamin C with DCPIP?
c Pipette 2 cm3 of vitamin C solution into a test tube. d Using a graduated pipette or a burette, add 1% DCPIP drop by drop to the vitamin C solution. Shake the tube gently after adding each drop. Add DCPIP solution until the blue colour of the final drop does not disappear.
What method is used to test for vitamin C content?
Direct ultraviolet spectrophotometry
Direct ultraviolet spectrophotometry is a fast, simple and reliable method for the determination of vitamin C.
How is the DCPIP test carried out?
Draw up some of the clear liquid into a pipette and then add it, one drop at a time, to a test tube containing a light blue solution of DCPIP. If the extract is acid the colour will change from blue to red. Continue to add more and see if the colour disappears altogether.
How do you test vitamin C content in fruits and vegetables?
You can test fruit at home to determine the amount of vitamin C it contains by using the vitamin C titration protocol. You can test fruit at home to determine the amount of vitamin C it contains by using the vitamin C titration protocol — a vitamin C experiment with iodine.
How do you isolate vitamin C?
Vitamin C is water soluble, so all you need do is crush or grind the plant material and then shake it up with water. A common method is to grind the material with some clean sand in a mortar with a pestle. The material can then be filtered or centrifuged to get rid of the debris. The vitamin C will be in the water.
How do you make a vitamin C indicator?
Measure one cup of water into a clean glass jar. Add one teaspoon of the cornstarch solution and four drops of iodine to the plain water. It will turn blue. This will be your indicator solution.
What is the function of DPIP in this experiment?
The function of the DPIP in this experiment is to act as the electron acceptor, replacing the usual NADP found in plants. When the light shines on the active chloroplasts, the electrons are excited, which causes them to jump to a higher energy level thus reducing the DPIP.