What is protoplast isolation and culture?
Protoplasts are cells which have had their cell wall removed, usually by digestion with enzymes. The isolation and culture media used vary with the species and with the tissue from which the protoplasts were isolated. Protoplasts are used in a number of ways for research and for plant improvement.
What is a protoplast culture?
The basic principle of protoplast culture is the aseptic isolation of large number of intact living protoplasts removing their cell wall and cultures them on a suitable nutrient medium for their requisite growth and development. Protoplast can be isolated from varieties of plant tissues.
What is protoplast culture PPT?
Introduction – The Protoplast ● Is a naked cell surrounded by a plasma membrane. It can regenerate cell wall, grow and divide. ● Is fragile but can be cultured and grow into a whole plant. ● Cells can originate from any type of tissue.
What are the different methods of protoplast isolation?
The enzymatic isolation of protoplasts can be performed in two different ways: Two step or sequential method: The tissue is first treated with a macerozyme or pectinase enzyme which isolates the cells by degrading the middle lamella. These free cells are then treated with cellulase which releases the protoplasts.
What is protoplast isolation culture and fusion?
Protoplasts are isolated by two methods, mechanical and enzymatic. Mechanical isolation method of protoplasts is no more practically used. It is only a historical method. This involves cutting of plasmolysed cells (in fact cell walls) with a sharp blade to release protoplasts.
What is protoplast isolation Slideshare?
Cut the tissue which are first plasmolysed with a sharp knife into small pieces. Then these pieces are deplasmolysed by using dilute solution to release the protoplasts. Generally protoplasts were isolated from highly vacuolated cells of storage tissues (onion bulbs, scales, radish root, beet root).
What is protoplast isolation and fusion?
Protoplast = cell- cell wall Protoplast are plant cell with the plasma membrane but without the cell wall. Protoplast allow the fusion of similar or different species and the fused product can generate into the whole plant.
Who is the father of tissue culture?
Gottlieb Haberlandt
Tissue culture is an artificial method involving in-vitro cultivation of plant cells, tissue, or organs in nutrient solutions under controlled lab conditions. It was first discovered in 1898 by a German botanist, Gottlieb Haberlandt.
What are the applications of protoplast culture?
Organelle Isolation: Protoplasts are very convenient material for the isolation of chloroplasts, mitochondria, nuclei and even chromosomes. It has been demonstrated that chloroplasts particularly isolated from cereal protoplast have higher capacity for CO2 fixation than those obtained by mechanical grinding.